luna universal probe qpcr master mix (New England Biolabs)

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New England Biolabs luna universal probe qpcr master mix
Luna Universal Probe Qpcr Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 756 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/luna+universal/Luna+Universal+Probe+qPCR+Master+Mix/pmc13125916-186-29-35
Average 99 stars, based on 756 article reviews

luna universal probe qpcr master mix - by Bioz Stars, 2026-09

99/100 stars

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Polymerase Chain Reaction:

Article Title: Multipurpose Passive Surveillance of Bat-Borne Viruses in Hungary: Lyssaviruses and Filoviruses in Focus
Article Snippet: .. First round of the PCR was used with the Luna Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswich, MA, USA) with the reaction setup as follows: at 55 °C for 10 min, and 95 °C for 1 min, followed by 30 cycles of 95 °C for 10s, 53 °C for 30s, 60 °C for 1 min, finally 60 °C for 6 min, and 4 °C ∞. ..

Quantitative RT-PCR:

Article Title: The Effects of Eyestalk Ablation on the Androgenic Gland and the Male Reproductive Organs in the Kuruma Prawn Marsupenaeus japonicus .
Article Snippet: .. Maj-IAG expression was analyzed using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs), and Maj-Dsx1 and Maj-Dsx2 were analyzed using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs). ..

Article Title: CPSF73 activation and 3' RNA polymerase II pausing are lost during readthrough transcription after heat shock.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Rpb1 N-term mAb (D8L4Y) Cell Signaling Cat#14958; RRID: AB_2687876 RNA Pol II CTD phospho Ser2 antibody (AbFlex rAb) Active Motif Cat#91115; RRID: AB_2793780 RNA Pol II CTD phospho Tyr1 antibody (AbFlex rAb) Active Motif Cat#91219; RRID: AB_2793809 Rabbit polyclonal anti-CPSF73 Antibody Bethyl Cat#A301-091A; RRID: AB_2084528 Mouse monoclonal anti-HA Antibody Generated in house 12CA5 Mouse monoclonal anti-GAPDH Antibody Santa Cruz Cat#sc-32233; RRID: AB_627679 Rabbit polyclonal anti-TBP Antibody Proteintech Cat#22006-1-AP; RRID: AB_10951514 Mouse monoclonal anti-RBBP6 antibody HRP conjugated Santa Cruz Cat#sc-9962; RRID: AB_2284969 Horse anti-mouse IgG antibody HRP conjugated Cell Signaling Cat#7076; RRID: AB_330924 Goat anti-rabbit IgG antibody Cell Signaling Cat#7074; RRID: AB_2099233 Critical commercial assays μMACS streptavidin kit Miltenyi Cat#130-133-282 Luna universal one step RT-qPCR kit NEB Cat#E3005 Luna universal qPCR kit NEB CatM3003 Deposited data 4sU-seq, ChIP-seq data This paper GSE303860 (https://www.ncbi.nlm.nih.gov/ geo/query/acc.cgi?acc=GSE303860) 4sU RNA-seq from RBBP6 depletion experiments Yoon et al. 8 GSE260584 (https://www.ncbi.nlm.nih.gov/ geo/query/acc.cgi?acc=GSE260584) Experimental models: Cell lines Human: HEK293: WT ATCC CRL-1573 Human: HEK293: pSBtet-mCherry This study N/A Human: HEK293: pSBtet-HA-N-RBBP6 This study N/A Human: HEK293: pSBtet-CPSF73 This study N/A Oligonucleotides See Table S1 for primer sequences IDT DNA N/A Recombinant DNA pSBtet-mCherry Liu et al. 49 N/A pSBtet-CPSF73 Liu et al. 49 N/A pCMV(CAT)T7-SB100 Liu et al. 49 Addgene Plasmid #34879 pSBtet-HA-N-RBBP6 This study N/A Software and algorithms HISAT2, v2.2.1 Kim et al. 50 http://daehwankimlab.github.io/hisat2/ Bowtie2, v2.4.2 Langmead and Salzberg 51 https://bowtie-bio.sourceforge.net/bowtie2/ index.shtml Homer, v4.10 Heinz et al. 52 http://homer.ucsd.edu/homer/ BedTools, v2.28.0 Quinlan et al. 53 https://bedtools.readthedocs.io/en/latest/index.html DESeq2, v1.34.0 Love et al. 54 https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R studio, v1.2.5033 Posit https://posit.co/downloads/ R, v4.1.2 R Project https://www.r-project.org GraphPad online calculators GraphPad https://www.graphpad.com/quickcalcs/contmenu/ Microsoft Excel Microsoft https://www.microsoft.com/en-us/ microsoft-365/excel Custom code This study https://zenodo.org/records/18262812 Cell Reports 45, 117039, March 24, 2026 13 .. HEK293 cells (ATCC CRL-1573) were used in this study.

Article Title: Detection of Bovine Viral Diarrhoea Virus in a Case Series of Clinically Cachectic Cattle from Tiaret, Algeria
Article Snippet: Nucleic acid was extracted from 200 μL of each serum sample using the IndiMag® Pathogen Kit (QIAGEN for INDICAL BIOSCIENCE, Leipzig, Germany) with the IndiMag 48s (INDICAL) extraction system. .. Amplification of viral RNA was conducted using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs® Ipswich, MA, USA) on an Applied Biosystems Quant Studio 3 Real-Time PCR System. ..

Article Title: Detection of Bovine Viral Diarrhoea Virus in a Case Series of Clinically Cachectic Cattle from Tiaret, Algeria
Article Snippet: Nucleic acid was extracted from 200 μL of each serum sample using the IndiMag ® Pathogen Kit (QIAGEN for INDICAL BIOSCIENCE, Leipzig, Germany) with the IndiMag 48s (INDICAL) extraction system. .. Amplification of viral RNA was conducted using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs ® Ipswich, MA, USA) on an Applied Biosystems Quant Studio 3 Real-Time PCR System. ..

Article Title: Stealth replication of SARS-CoV-2 Omicron in the nasal epithelium at physiological temperature.
Article Snippet: .. Culture apical supernatants collected at 1, 2, and 4 dpi were inactivated for 20 min at 80°C and then subjected to RT-qPCR analysis, using a Luna Universal One-Step RT-qPCR Kit (New England Biolabs), following the manufacturer’s instructions. .. SARS-CoV-2 RNA was quantified in a final volume of 5 μL per reaction in 384-well plates using SARS-CoV-2 Nspecific primers (Forward 5′-CGA AGG TGT GAC TTC CAT G-3′; Reverse 5′- TAA TCA GAC AAG GAA CTG ATT A-3′) on a QuantStudio 6 Flex thermocycler (Applied Biosystems).

Article Title: The Effects of Eyestalk Ablation on the Androgenic Gland and the Male Reproductive Organs in the Kuruma Prawn Marsupenaeus japonicus
Article Snippet: .. Maj-IAG expression was analyzed using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs), and Maj-Dsx1 and Maj-Dsx2 were analyzed using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs). ..

Article Title: Virological characterization of SARS-CoV-2 BA.2.86 variants by assessing antiviral susceptibility, in vivo infectivity and replicative fitness
Article Snippet: .. Detection of viral genomes from heat-inactivated samples was performed by RT-qPCR using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) with SARS-CoV-2 specific primers targeting the N gene region (5′-TAATCAGACAAGGAACTGATTA-3′ and 5′-CGAAGGTGTGACTTCCATG-3′) and with the following cycling conditions: 55 °C for 10 min, 95 °C for 1 min, for 1 cycle, followed by 95 °C for 10 s, 60 °C for 1 min, for 40 cycles in an Applied Biosystems QuantStudio 6 thermocycler. ..

Expressing:

Generated:

Real-time Polymerase Chain Reaction:

Article Title: Detection of Bovine Viral Diarrhoea Virus in a Case Series of Clinically Cachectic Cattle from Tiaret, Algeria
Article Snippet: Nucleic acid was extracted from 200 μL of each serum sample using the IndiMag ® Pathogen Kit (QIAGEN for INDICAL BIOSCIENCE, Leipzig, Germany) with the IndiMag 48s (INDICAL) extraction system. .. Amplification of viral RNA was conducted using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs ® Ipswich, MA, USA) on an Applied Biosystems Quant Studio 3 Real-Time PCR System. ..

Chromatin Immunoprecipitation:

RNA Sequencing:

Recombinant:

Plasmid Preparation:

Software:

Amplification:

Article Title: Detection of Bovine Viral Diarrhoea Virus in a Case Series of Clinically Cachectic Cattle from Tiaret, Algeria
Article Snippet: Nucleic acid was extracted from 200 μL of each serum sample using the IndiMag ® Pathogen Kit (QIAGEN for INDICAL BIOSCIENCE, Leipzig, Germany) with the IndiMag 48s (INDICAL) extraction system. .. Amplification of viral RNA was conducted using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs ® Ipswich, MA, USA) on an Applied Biosystems Quant Studio 3 Real-Time PCR System. ..

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